ba 2000 (Vector Laboratories)

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Vector Laboratories ba 2000
Ba 2000, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 7578 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/BA-2000-1%2E5/Biotinylated+Horse+Anti-Mouse+IgG+Antibody/pmc13641933-71-45-44
Average 96 stars, based on 7578 article reviews

ba 2000 - by Bioz Stars, 2026-11

96/100 stars

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Virus:

Article Title: Reduced dorsal CA1 activity limits retention of the temporal component of declarative memory in the Cntnap2 knockout mouse model of autism
Article Snippet: .. Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (Mus musculus) B6.129(Cg)-Cntnap2tm1Pele/J Jackson #017482; RRID:IMSR_ JAX:017482 Males and females Strain, strain background (Adeno- associated Virus) AAV5- pCaMKIIα-ArchT- GFP UNC Vector Core RRID:Addgene_99039AAV5 Strain, strain background (Adeno- associated Virus) AAV5- pCaMKIIα-ChR2 (H134R)- EYFP UNC Vector Core RRID:Addgene_26969 Strain, strain background (Adeno- associated Virus) AAV5- pCaMKIIα-GFP UNC Vector Core RRID:Addgene_50469 Strain, strain background (Adeno- associated Virus) AAV1- hsynjGCaMP6f- WPRE Addgene RRID:Addgene_100837 Antibody Mouse monoclonal anti- cFos Santa Cruz SC166940; RRID:AB_2106783 1:1000 Antibody Mouse polyclonal anti- Biotin Vector Laboratories BA- 2000; RRID:AB_2313581 1:200 Antibody, fluorophore Streptavidin 647 Jackson 016- 600- 084; RRID:AB_2341101 1:200 Software, algorithm Statview N/A N/A Software, algorithm Fiji ImageJ https://imagej.net/ software/fiji/downloads Software, algorithm Bonsai/Neurophotometrics FP3002 Software, algorithm Qu Path RRID:SCR_018257 Software, algorithm GraphPad Prism RRID:SCR_002798 Other Fear Conditioning FEAR- SHUTTLE Fear-Shuttle - Imetronic Behavioral apparatus Other Stereotactic frame Kopf Instruments N/A Stereotaxic surgery apparatus Other Optical fiber stubs 2 mm thorlabs N/A Optic fiber implants for fiberphotometry and optogenetic experiments Other LED driver system Thorlabs LEDD1B T- Cube LED for optogenetic stimulation Other Fiber- Coupled LED Thorlabs M470F4 LED for fiberphotometry recordings Other vertical P10 puller Narishige, Japan N/A Puller for glass pipette preparation for viral injection Other Glass Capillary, Borosilicate Clarke Electromedical GC- 150F- 15 Glass pipette for viral injection Other vibratome VT1200 LEICA N/A Slicer for fixed brain tissue for immunohistochemistry Other Slide scanner Axioscan Z1 Zeiss N/A Epifluorescence microscope for imaging ..

Software:

Fear Conditioning:

Optogenetics:

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Immunohistochemistry:

Microscopy:

Imaging:

Incubation:

Article Title: Data-driven thresholds for standardized classification of severe Alzheimer's disease neuropathology using digital neuropathology.
Article Snippet: .. The following day, sections were washed twice in 1 TBS (5 min each) and incubated for 1 h at room temperature with a biotinylated horse anti-mouse secondary antibody (Vector Laboratories, Cat #BA-2000, 1:250). .. After two additional TBS washes, slides were treated for 1 h with the VECTASTAIN® Elite ABC Peroxidase kit (Vector Laboratories, Cat #PK-6100), prepared according to manufacturer instructions.

Article Title: Recurrent Sleep Restriction During Aging Induces Early Recognition Memory Impairment, Neuroinflammation, and Blood–Brain Barrier Dysfunction in Female Wistar Rats
Article Snippet: Membranes were then incubated overnight at 4 °C with the following primary antibodies: ZO-1 (Invitrogen, Cat. No. 40–2200, 1:500), occludin (Invitrogen, Cat. No. 40–4700, 1:500), and β-actin (Santa Cruz Biotechnology, Cat. No. sc-69879, 1:2500). .. After three washes, PVDF membranes were incubated with the secondary antibodies biotinylated anti-rabbit IgG (Vector Laboratories, #BA-1000, 1:2000) and biotinylated anti-mouse IgG (Vector Laboratories, #BA-2000, 1:2000) for 2 h with constant shaking at room temperature. .. After three more washes, membranes were incubated with an ABC kit (Vectastain PK-6100) for 30 min.

Article Title: Complementary PET and MRI of myeloperoxidase activity as a noninvasive surrogate for tissue acrolein in experimental neuroinflammation
Article Snippet: Duplicate gels were stained with Coomassie blue (ThermoScientific, 20278) for normalization. .. Proteins were transferred to nitrocellulose membrane using Power Blotter-Semi-dry Transfer System (Thermo Fisher Scientific) which was blocked with 1x casein (Vector, #SP-5020) for 30 min, incubated overnight (4 °C) with anti-acrolein-lysine antibody (StressMarq, SMC-505, 1:1000), washed with 0.1% TBST, and incubated with secondary antibodies (Vector, #BA-2000, 1:2000) for 45 min. .. Following TBST washing, the membrane was treated with VECTASTAIN ABC-AmP reagent (Vector Laboratories) for 10 min. DuoLuX substrate (Vector, #SK-6605) was used for chemiluminescent detection on an Azure C300 Western blot imaging system (Azure Biosystems).

Article Title: Incident versus prevalent progressive supranuclear palsy: analyses of U.S. Alzheimer's Disease Research Centers data indicate clinical and neuropathological features of a relatively common and underrecognized tauopathy in the aging brain.
Article Snippet: .. The following day, slides were washed twice in 1× TBS for 5 min each and then incubated for 1 h at room temperature with biotinylated horse anti-mouse secondary antibody (Vector Laboratories, #BA-2000, 1:250). .. After two additional 1× TBS washes, sections were incubated for 1 h with the ABC Peroxidase (VECTASTAIN Elite ABC Peroxidase kit, Vector Laboratories, #PK-6100).

Staining:

Article Title: Vitamin D 3 -induced changes in steroidogenic pathway within periovarian adipose tissue of rats with polycystic ovary syndrome*
Article Snippet: .. Next, the antigens were visualized using biotinylated secondary antibodies (1.5 h at RT) anti-mouse (1:300; cat. no. BA-2000; Vector Laboratories) or anti-rabbit (1:300; cat. no. BA-1000; Vector Laboratories), avidinbiotin-peroxidase complex (40 min at RT; Vectastain Elite ABC Reagent, Vector Laboratories), and 3,3’-diaminobenzidine (DAB; Sigma-Aldrich) as chromogen staining substrate. ..

Comparison:

Article Title: Achieving cell-type specific transduction with adeno-associated viral vectors in pigeons
Article Snippet: ms anti-NeuN 1:500 , Thermo Fisher Scientific Cat# MA5-33103, RRID: AB_2802653 , gt anti-mouse Alexa Fluor 488 1:250 , Thermo Fisher Scientific Cat# A-11001, RRID: AB_2534069. .. Comparison of AAV Serotypes for Anterograde and Retrograde Transport , ms anti-ChR2 1:1000 , Progen Cat#651180, RRID: AB_2892521 , biotinylated horse anti-mouse IgG 1:500 , Vector Laboratories Cat# BA-2000, RRID: AB_2313581. .. rb anti-RFP 1:1000 , Antibodies-Online Cat# ABIN129578, RRID: AB_10781500 , gt anti-rabbit Alexa Fluor 594 1:500 , Thermo Fisher Scientific Cat# A32740, RRID: AB_2762824.

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